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KMID : 0364819990350010013
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Korean Journal of Microbiology
1999 Volume.35 No. 1 p.13 ~ p.17
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Cloning and Nucleotide Sequence Analysis of the asd Gene from Shigella sonnei KNIH104S
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Abstract
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Shigella sonnei is important causes of human enteric infections. S. sonnei KNIH104S was isolated from patient of shigellosis in Korea and previously reported. We cloned 1.7 kb BamHI fragment containing the asd gene encoding an aspartate β-semialdehyde dehydrogenase from chromosomal DNA of S. sonnei KNIH104S. This recombinant
plasmid was named as pSAB17. E. coli χ6097, and asd- mutant, cannot grow on the LB medium without DL-α, ε-diaminopimelic acid (50 ㎍/ml) but E. coli χ6097(pSAB17) can grow on the same medium. We sequenced the asd gene of Shigella for the first time. The asd gene was composed of 1,104 base pairs with ATG initiation codon and TAA termination codon. Sequence comparison of the asd gene exhibited 99.9% nucleotide sequence homology with that of E. coli. Also, We constructed the balanced-lethal vector using pBluescrip SK(+) and asd gene of S. sonnei KNIH104S.
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KEYWORD
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asd gene, balanced-lethal vector, Shigella sonnei KNIH104S,
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